Measurement

Part:BBa_J100091:Experience

Designed by: Malcolm Campbell and Todd Eckdahl   Group: Campbell M Lab   (2012-08-20)

pClone Basic contains a double transcriptional terminator flanked by BsaI sites that use CGAC and GCGG sticky ends. Downstream of the BsaI sites are a ribosome binding site (RBS) and a RFP reporter gene. A pClone Basic negative control assembly produces colonies lacking RFP fluorescence under UV light (left). GGA with a functional promoter produces red fluorescent colonies (center), but GGA with a non-functional promoter yields colonies with the normal pale color (right).

PCloneBasic.png

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